rabbit polyclonal  (Alomone Labs)

Journal: bioRxiv

Article Title: Differential contribution of HCN1 and HCN4 to the synchronisation of sinoatrial pacemaker cells

doi: 10.64898/2025.12.16.694405

Figure Lengend Snippet: The SAN network forms the intrinsic leading pacemaker region of the heart driven by If-conducting cells. (A) Preparation of a gelatine-inflated heart indicating the SAN region (dashed line). (B) Fluorescence imaging of an anti-HCN1 (red signal) and anti-HCN4 (green signal) immunostained whole-mount right atrial preparation of a WT heart, revealing the anatomical extension (head, body, tail region) and spatial HCN1/HCN4 channel expression profile of the SAN. (C) Isolated SAN pacemaker cell (elongated cell) during a patch clamp recording. (D) Family of current traces (If) recorded from a WT pacemaker cell of the sinoatrial node using the voltage protocol shown on the top. From a holding potential of −50 mV, a series of voltage pulses ranging from −140 to - 20 mV (duration 5 s, delta V: 15 mV) were applied. (E) Mean activation curve of native If recorded from WT SAN pacemaker cells (n=13). Additional abbreviations: CT, crista terminalis; SVC, superior vena cava.

Article Snippet: After permeabilization (0.5% Triton X100, 20% DMSO in PBS) and blocking in 5% NDS (Normal Donkey Serum), the tissue was incubated with guinea-pig polyclonal HCN1 antibody (1:500; Alomone Labs, Jerusalem, Israel) and rabbit polyclonal HCN4 antibody (1:500; Alomone Labs).

Techniques: Fluorescence, Imaging, Expressing, Isolation, Patch Clamp, Activation Assay

Journal: The Journal of Physiological Sciences : JPS

Article Title: Doxorubicin-induced sinus node dysfunction associated with mitochondria and nuclear impairment in a mouse model

doi: 10.1016/j.jphyss.2025.100047

Figure Lengend Snippet: Doxorubicin-induced cardiomyopathy modelling in mice. A. A work flow of the modelling of doxorubicin (DXB)-induce cardiomyopathy in C57BL/6 N adult male mice. Saline was injected as a vehicle (Veh) control. B. The time course of the body weight in Veh- and DXB-treated mice. While the body weight of Veh mice incrementally gained, that of DXB mice decreased. n = 6 in Veh and 10 in DXB. C. Heart weight-to-tibial length ratio in Veh- and DXB-treated mice. n = 6 in Veh and 5 in DXB. D and E. Picrosirius red-stained sections (D) and averaged fibrosis area (E) of the left ventricular myocardium in Veh- and DXB-treated mice. n = 4/group. Scale bars = 20 μm. F. Representative picrosirius red-stained sections of the SN region in Veh- or DXB-treated mice and enlarged area labelled with HCN4, a pacemaker channel as a SN marker, indicated by rectangles in picrosirius red images. Scale bars = 100 μm. G. Averaged fibrosis area of the SN region in Veh- and DXB-treated mice. n = 4/group. * p < 0.05 vs Veh, determined by unpaired t -test.

Article Snippet: After blocking with 0.1 % bovine serum albumin in PBS, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-HCN4 antibodies (1:100, APC-052, Alomone Labs).

Techniques: Saline, Injection, Control, Staining, Marker

Journal: The Journal of Physiological Sciences : JPS

Article Title: Doxorubicin-induced sinus node dysfunction associated with mitochondria and nuclear impairment in a mouse model

doi: 10.1016/j.jphyss.2025.100047

Figure Lengend Snippet: Chronic effect of doxorubicin (DXB) on expression levels of genes responsible for pacemaking. A and B. Gene expression levels of HCN4 pacemaker channel and Ca 2+ regulators in the sinus node (A) and the right atrial myocardium (B) in vehicle (Veh)- and DXB-treated mice. n = 6 in SN, n = 3 in right atrium, * p < 0.05 vs Veh determined by unpaired t -test.

Article Snippet: After blocking with 0.1 % bovine serum albumin in PBS, the sections were incubated overnight at 4 °C with rabbit polyclonal anti-HCN4 antibodies (1:100, APC-052, Alomone Labs).

Techniques: Expressing, Gene Expression